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首頁 > 蛋白生物學(xué) > 蛋白提取純化 > 動物蛋白提取

柱式動物總蛋白提取試劑盒（細胞樣品，變性提?。? width="255px" /></a><input type="hidden" name="hidProductID" id="hidProductID" value="1326" /><input type="hidden" name="HidProductName" id="HidProductName" value="柱式動物總蛋白提取試劑盒（細胞樣品，變性提?。? /><input type="hidden" name="HidRetailPrice" id="HidRetailPrice" value="500" /><input type="hidden" name="HidColors" id="HidColors" value="50次" />
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<h3>柱式動物總蛋白提取試劑盒（細胞樣品，變性提?。?/h3>
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<p>產(chǎn)品編號：RTD8302</p>
<p>產(chǎn)品規(guī)格：50次</p>
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<span>數(shù)量</span>
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<span>價格</span>
<span>￥500</span>
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<div><p class="MsoNormal" align="center" style="text-align:center;">
<b>柱式動物總蛋白提取試劑盒（細胞樣品，變性提取）</b><b> </b>
</p>
<p class="MsoNormal" align="center" style="text-align:center;">
<b>Column Animal Cells Denaturing Total Protein
Extraction Kit </b><b>Ver.740164</b><b></b>
</p>
<div align="center">
<table class="MsoNormalTable" border="1" cellspacing="0" cellpadding="0" width="466" style="width:349.35pt;border:none;">
<tbody>
<tr>
<td width="80" valign="top" style="border:solid windowtext 1.0pt;background:#E0E0E0;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;background:#D9D9D9;">貨號</span></b><b><span style="font-family:"background:#D9D9D9;"></span></b>
</p>
</td>
<td width="327" valign="top" style="border:solid windowtext 1.0pt;background:#E0E0E0;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;background:#D9D9D9;">產(chǎn)品名稱</span></b><b><span style="font-family:"background:#D9D9D9;"></span></b>
</p>
</td>
<td width="59" valign="top" style="border:solid windowtext 1.0pt;background:#E0E0E0;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;background:#D9D9D9;">規(guī)格</span></b><b><span style="font-family:"background:#D9D9D9;"></span></b>
</p>
</td>
</tr>
<tr>
<td width="80" valign="top" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">RTD8302</span>
</p>
</td>
<td width="327" valign="top" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:宋體;">柱式動物總蛋白提取試劑盒（細胞樣品，變性提?。?/span><span style="font-family:""></span>
</p>
</td>
<td width="59" valign="top" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">50</span><span style="font-family:宋體;">次</span><span style="font-family:""></span>
</p>
</td>
</tr>
</tbody>
</table>
</div>
<p class="MsoNormal">
<b>● </b><b>產(chǎn)品包裝：<span></span></b>
</p>
<div align="center">
<table class="MsoNormalTable" border="1" cellspacing="0" cellpadding="0" width="457" style="width:343.05pt;border:none;">
<tbody>
<tr>
<td width="94" valign="top" style="border:solid windowtext 1.0pt;background:#E0E0E0;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;background:#D9D9D9;">貨號</span></b><b><span style="font-family:"background:#D9D9D9;"></span></b>
</p>
</td>
<td width="244" valign="top" style="border:solid windowtext 1.0pt;background:#E0E0E0;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;background:#D9D9D9;">組份</span></b><b><span style="font-family:"background:#D9D9D9;"></span></b>
</p>
</td>
<td width="56" valign="top" style="border:solid windowtext 1.0pt;background:#E0E0E0;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;background:#D9D9D9;">規(guī)格</span></b><b><span style="font-family:"background:#D9D9D9;"></span></b>
</p>
</td>
<td width="63" valign="top" style="border:solid windowtext 1.0pt;background:#E0E0E0;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;background:#D9D9D9;">貯存</span></b><b><span style="font-family:"background:#D9D9D9;"></span></b>
</p>
</td>
</tr>
<tr>
<td width="94" valign="top" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">RTD8302-01</span>
</p>
</td>
<td width="244" valign="top" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:宋體;">變性裂解緩沖液</span><span style="font-family:""></span>
</p>
</td>
<td width="56" valign="top" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">25 ml</span>
</p>
</td>
<td width="63" valign="top" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">4</span><span style="font-family:宋體;">℃</span><span style="font-family:""></span>
</p>
</td>
</tr>
<tr>
<td width="94" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-size:11.0pt;font-family:"">CD-50</span>
</p>
</td>
<td width="244" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-size:11.0pt;font-family:宋體;">離心柱套裝</span><span style="font-size:11.0pt;font-family:""></span>
</p>
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-size:11.0pt;font-family:宋體;">（包含離心柱和</span><span style="font-size:11.0pt;font-family:"">2 ml</span><span style="font-size:11.0pt;font-family:宋體;">連蓋收集管）</span><span style="font-size:11.0pt;font-family:""></span>
</p>
</td>
<td width="56" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-size:11.0pt;font-family:"">50</span><span style="font-size:11.0pt;font-family:宋體;">套</span><span style="font-size:11.0pt;font-family:""></span>
</p>
</td>
<td width="63" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-size:11.0pt;font-family:"">RT</span>
</p>
</td>
</tr>
</tbody>
</table>
</div>
<p class="MsoNormal">
<b>● </b><b>產(chǎn)品簡介：<span></span></b>
</p>
<p class="MsoNormal" style="text-indent:21.0pt;">
本試劑盒可從動物懸浮細胞或貼壁細胞中提取總蛋白，用變性裂解緩沖液配合離心柱裂解細胞，無需超聲處理打斷DNA，提取過程簡便高效，大約10分鐘即可完成蛋白提取，可以提取到細胞內(nèi)的絕大多數(shù)蛋白質(zhì)（包括膜，細胞器，核，核基質(zhì)和胞質(zhì)蛋白）。獲得的總蛋白可用于SDS-PAGE
Western Blot檢測、免疫沉淀（IP）等后續(xù)研究。由于裂解緩沖液中含有SDS等強變性劑，不建議用于免疫共沉淀（Co-IP）、EMSA以及酶活性分析等與蛋白活性相關(guān)的實驗。
</p>
<p class="MsoNormal" style="text-indent:21.0pt;">
本產(chǎn)品提取的是細胞樣品的變性總蛋白，包括質(zhì)膜，細胞器，細胞核，核基質(zhì)和細胞質(zhì)內(nèi)的蛋白，提取的蛋白不適用非變性電泳。
</p>
<p class="MsoNormal" style="text-indent:21.0pt;">
按照每次使用~5×10<sup>6</sup>培養(yǎng)細胞加0.5 ml裂解緩沖液計算，本試劑盒大約可以使用50 次。
</p>
<p class="MsoNormal">
<b>● </b><b>保存、效期及運輸：<span></span></b>
</p>
<p class="MsoNormal" align="left" style="margin-left:22.65pt;">
按照標(biāo)簽溫度貯存；有效期一年；試劑盒常溫運輸。
</p>
<p class="MsoNormal">
<b>● </b><b>用前必讀：</b><b></b>
</p>
<p class="MsoNormal" style="margin-left:33.7pt;text-indent:-15.75pt;">
1.
離心機請調(diào)整成RCF/g模式，按照離心力設(shè)置離心機（不要根據(jù)轉(zhuǎn)速rpm模式設(shè)置），需要離心機轉(zhuǎn)速能達到16000
g（約20000
rpm），所有離心步驟都需要在4℃低溫離心機中進行。
</p>
<p class="MsoNormal" style="margin-left:18.0pt;">
2.
變性裂解緩沖液混勻后放置于冰上。將離心柱套裝蓋好管蓋，放置于冰上預(yù)冷。
</p>
<p class="MsoNormal" style="margin-left:33.7pt;text-indent:-15.75pt;">
3.
蛋白提取推薦添加蛋白酶抑制劑（自備，試劑盒不提供），根據(jù)蛋白酶抑制劑母液濃度提前添加在裂解緩沖液中（添加時按照1:100添加，即1
ml裂解緩沖液添加10
μl抑制劑）；研究蛋白磷酸化，需要添加磷酸酶抑制劑（自備，試劑盒不提供）。
</p>
<p class="MsoNormal" align="left" style="text-indent:15.75pt;">
4.
蛋白定量推薦使用BCA方法，可以選擇BCA蛋白濃度測定試劑盒（貨號：RTP7102）。<b></b>
</p>
<p class="MsoNormal" align="left">
<b>● </b><b>使用說明：<span></span></b>
</p>
<p class="MsoNormal" align="left" style="margin-left:19.05pt;text-indent:-15.8pt;">
<b>一</b><b>. </b><b>準備即用型裂解緩沖液：</b><b></b>
</p>
<p class="MsoNormal" align="left" style="margin-left:15.75pt;text-indent:21pt;">
取適當(dāng)體積的預(yù)冷裂解緩沖液，在使用前數(shù)分鐘內(nèi)加入1/100體積的蛋白酶抑制劑（100×）（自備，試劑盒不提供），如果進行蛋白磷酸化研究，需要加入1/100體積的磷酸酶抑制劑
</p>
<p class="MsoNormal" align="left" style="margin-left:15.75pt;text-indent:21pt;">
（100×）（自備，試劑盒不提供），隨后立即放于冰上待用。
</p>
<p class="MsoNormal" align="left" style="margin-left:13.8pt;text-indent:-10.55pt;">
<b>二</b><b>. </b><b>貼壁細胞蛋白提取：</b><b></b>
</p>
<p class="MsoNormal" align="left" style="margin-left:29.2pt;text-indent:-15.45pt;">
2.1 將預(yù)冷的 <span>1</span>×PBS直接加入培養(yǎng)板，培養(yǎng)皿或培養(yǎng)瓶中清洗貼壁細胞，吸去上清。
</p>
<p class="MsoNormal" align="left" style="margin-left:29.2pt;text-indent:-15.45pt;">
2. 2 按照下表中將相應(yīng)體積的變性裂解緩沖液均勻的加入整個器皿表面，用移液器吹打混勻，冰浴5分鐘。
</p>
<div align="center">
<table class="MsoNormalTable" border="1" cellspacing="0" cellpadding="0" width="600" style="width:450.15pt;border:none;">
<tbody>
<tr>
<td width="89" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;">細胞類型</span></b><b><span style="font-family:""></span></b>
</p>
</td>
<td width="112" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;">培養(yǎng)器皿</span></b><b><span style="font-family:""></span></b>
</p>
</td>
<td width="86" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;">細胞數(shù)量</span></b><b><span style="font-family:""></span></b>
</p>
</td>
<td width="170" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;">細胞沉淀體積（</span></b><b><span style="font-family:"">PCV</span></b><b><span style="font-family:宋體;">）（</span></b><b><span style="font-family:"">μl</span></b><b><span style="font-family:宋體;">）</span></b><span style="font-family:""></span>
</p>
</td>
<td width="143" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;">裂解緩沖液（</span></b><b><span style="font-family:"">μl</span></b><b><span style="font-family:宋體;">）</span></b><b><span style="font-family:""></span></b>
</p>
</td>
</tr>
<tr>
<td width="89" rowspan="7" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:宋體;">貼壁細胞</span><span style="font-family:""></span>
</p>
</td>
<td width="112" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">24</span><span style="font-family:宋體;">孔板</span><span style="font-family:""></span>
</p>
</td>
<td width="86" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">~5×10<sup>5</sup></span>
</p>
</td>
<td width="170" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">~5</span>
</p>
</td>
<td width="143" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">50</span>
</p>
</td>
</tr>
<tr>
<td width="112" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">6</span><span style="font-family:宋體;">孔板</span><span style="font-family:""></span>
</p>
</td>
<td width="86" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:Arial, sans-serif;">~2.5×10<sup>6</sup></span><span style="font-family:""></span>
</p>
</td>
<td width="170" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">~25</span>
</p>
</td>
<td width="143" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">200</span>
</p>
</td>
</tr>
<tr>
<td width="112" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">25cm<sup>2</sup></span><span style="font-family:宋體;">培養(yǎng)瓶</span><span style="font-family:""></span>
</p>
</td>
<td width="86" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:Arial, sans-serif;">~2×10<sup>6</sup></span><span style="font-family:""></span>
</p>
</td>
<td width="170" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">~20</span>
</p>
</td>
<td width="143" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">200</span>
</p>
</td>
</tr>
<tr>
<td width="112" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">75cm<sup>2</sup></span><span style="font-family:宋體;">培養(yǎng)瓶</span><span style="font-family:""></span>
</p>
</td>
<td width="86" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">~8×10<sup>6</sup></span>
</p>
</td>
<td width="170" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">~80</span>
</p>
</td>
<td width="143" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">500</span>
</p>
</td>
</tr>
<tr>
<td width="112" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:Arial, sans-serif;">35
mm</span><span style="font-family:宋體;">培養(yǎng)皿</span><span style="font-family:""></span>
</p>
</td>
<td width="86" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:Arial, sans-serif;">~2×10<sup>6</sup></span><span style="font-family:""></span>
</p>
</td>
<td width="170" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">~20</span>
</p>
</td>
<td width="143" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">200</span>
</p>
</td>
</tr>
<tr>
<td width="112" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:Arial, sans-serif;">60
mm</span><span style="font-family:宋體;">培養(yǎng)皿</span><span style="font-family:Arial, sans-serif;"></span>
</p>
</td>
<td width="86" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:Arial, sans-serif;">~5×10<sup>6</sup></span><span style="font-family:""></span>
</p>
</td>
<td width="170" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">~50</span>
</p>
</td>
<td width="143" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">500</span>
</p>
</td>
</tr>
<tr>
<td width="112" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:Arial, sans-serif;">100
mm</span><span style="font-family:宋體;">培養(yǎng)皿</span><span style="font-family:Arial, sans-serif;"></span>
</p>
</td>
<td width="86" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:Arial, sans-serif;">~1.5×10<sup>7</sup></span><span style="font-family:""></span>
</p>
</td>
<td width="170" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:宋體;">調(diào)整細胞數(shù)目到</span><span style="font-family:"">2-5×10<sup>6</sup></span>
</p>
</td>
<td width="143" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">200</span>
</p>
</td>
</tr>
</tbody>
</table>
</div>
<p class="MsoNormal" align="left" style="margin-left:29.2pt;text-indent:-15.45pt;">
注： (二百萬，2×10<sup>6</sup>)HeLa細胞，其細胞沉淀體積（PCV，Packed Cell Volume）大約為20 μl。
</p>
<p class="MsoNormal" align="left" style="margin-left:29.2pt;text-indent:-15.45pt;">
2. 3 將裂解的細胞轉(zhuǎn)移到預(yù)冷的離心柱中，4℃ 16000g 離心 <span>1</span>分鐘。 <span></span>
</p>
<p class="MsoNormal" align="left" style="margin-left:29.2pt;">
注：裂解中細胞會釋放出變性的核酸，呈團狀粘稠透明樣，如移液器不易吸取，可以將吸頭剪尖，保證將全部裂解物加到離心柱中。
</p>
<p class="MsoNormal" align="left" style="margin-left:29.2pt;text-indent:-15.45pt;">
2. 4 棄去離心柱，將收集管中蛋白溶液轉(zhuǎn)移至干凈的 1.5 ml
離心管中，即可用于蛋白濃度測定和后續(xù)實驗。注：如果收集管中有少許沉淀，只需收集上清即可。
</p>
<p class="MsoNormal" align="left" style="margin-left:13.8pt;text-indent:-10.55pt;">
<b>三</b><b>. </b><b>懸浮細胞蛋白提取：</b><b></b>
</p>
<p class="MsoNormal" align="left" style="margin-left:34.15pt;text-indent:-20.6pt;">
3.1 400 g 4℃ 離心5 min收集細胞；用1 ml 1×PBS重懸細胞，400 g 4℃ 離心5 min收集細胞；重復(fù)漂洗細胞一次；留下細胞沉淀備用。
</p>
<p class="MsoNormal" align="left" style="margin-left:34.15pt;text-indent:-20.6pt;">
3.2 按照下表加入相應(yīng)體積的變性裂解緩沖液，用移液器重懸，冰浴處理5分鐘。
</p>
<div align="center">
<table class="MsoNormalTable" border="1" cellspacing="0" cellpadding="0" width="493" style="width:369.45pt;border:none;">
<tbody>
<tr>
<td width="76" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;">細胞類型</span></b><b><span style="font-family:""></span></b>
</p>
</td>
<td width="86" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;">細胞數(shù)量</span></b><b><span style="font-family:""></span></b>
</p>
</td>
<td width="179" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;">細胞沉淀體積（</span></b><b><span style="font-family:"">PCV</span></b><b><span style="font-family:宋體;">）（</span></b><b><span style="font-family:"">μl</span></b><b><span style="font-family:宋體;">）</span></b><span style="font-family:""></span>
</p>
</td>
<td width="151" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<b><span style="font-family:宋體;">裂解緩沖液（</span></b><b><span style="font-family:"">μl</span></b>
</p>
</td>
</tr>
<tr>
<td width="76" rowspan="3" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:宋體;">懸浮細胞</span><span style="font-family:""></span>
</p>
</td>
<td width="86" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">~1</span><span style="font-family:宋體;">×</span><span style="font-family:"">10<sup>6</sup></span>
</p>
</td>
<td width="179" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">~10</span>
</p>
</td>
<td width="151" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">100</span>
</p>
</td>
</tr>
<tr>
<td width="86" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">~2</span><span style="font-family:宋體;">×</span><span style="font-family:"">10<sup>6</sup></span>
</p>
</td>
<td width="179" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">~20</span>
</p>
</td>
<td width="151" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">200</span>
</p>
</td>
</tr>
<tr>
<td width="86" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">~5-10</span><span style="font-family:宋體;">×</span><span style="font-family:"">10<sup>6</sup></span>
</p>
</td>
<td width="179" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:宋體;">＞</span><span style="font-family:"">50</span>
</p>
</td>
<td width="151" style="border:solid windowtext 1.0pt;">
<p class="MsoNormal" align="center" style="text-align:center;">
<span style="font-family:"">500</span>
</p>
</td>
</tr>
</tbody>
</table>
</div>
<p class="MsoNormal" align="left" style="margin-left:29.2pt;text-indent:-15.45pt;">
注： (二百萬，2×10<sup>6</sup>)HeLa細胞，其細胞沉淀體積（PCV，Packed Cell Volume）大約為20 μl。
</p>
<p class="MsoNormal" align="left" style="margin-left:29.2pt;text-indent:-15.45pt;">
3. 3 將裂解的細胞轉(zhuǎn)移到預(yù)冷的離心柱中，4℃ 16000g 離心 <span>1</span>分鐘。 <span></span>
</p>
<p class="MsoNormal" align="left" style="margin-left:29.2pt;">
注：裂解中細胞會釋放出變性的核酸，呈團狀粘稠透明樣，如移液器不易吸取，可以將吸頭剪尖，保證將全部裂解物加到離心柱中。
</p>
<p class="MsoNormal" align="left" style="margin-left:34.15pt;text-indent:-20.6pt;">
3. 4 棄去離心柱，將收集管中蛋白溶液轉(zhuǎn)移至干凈的 1.5 ml
離心管中，即可用于蛋白濃度測定和后續(xù)實驗。注：如果收集管中有少許沉淀，只需收集上清即可。
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